Effects of Extract Endophytic Fungi Aspergillus sp. in Denture Adhesives of Acrylic Resins Related to the Surface Roughness and Candida Albicans Virulence.

ABSTRACT
Antifungal treatment is currently a serious problem because resistance and attachment of C. albicans often occur and can have an effect on the acrylic resin surface. One of them is adding antifungal concentration from the extract of endophyte fungi Aspergillus sp. contains chemical compounds that can inhibit C. albicans. The study aimed was to effect of extract of endophyte fungi Aspergillus sp. in denture adhesives of acrylic resins related to the surface roughness and C.albicans virulence. The research method was to extract the endophytic fungi Aspergillus sp. bio-tolerance test and measure the surface roughness of acrylic resin. The research sample used heat polymerized acrylic resin, namely the denture adhesive treatment group with a predetermined concentration, the control group using commercial denture adhesive and, added nystatin, each group suspended C. albicans for 24, 48 and 72 hours. Analysis using ANOVA. Bio-tolerance activity of Aspergillus sp. to C. albicans was the best at concentrations of 12.5% and 25%, the strongest occurred at 24 hours of incubation. The lower the concentration, the better it is to prevent surface roughness of the acrylic resin, but there is no difference in the surface roughness of acrylic resin concerning concentration and incubation time. Aspergillus sp. endophytic fungi extract. on denture adhesives can inhibit the activity and interaction of C. albicans as well as the surface roughness of acrylic resin which is better than synthetic antifungals.

Keywords: Extract Aspergillus sp., Denture adhesive, Bio-tolerance, C. albicans, Surface roughness