PHYSICAL RESPONSE OF ACRYLIC RESIN IN EFFECT OF Ziziphus mauritiana LAM RELATED TO GROWTH AND BIOFILM FORMATION OF Candida albicans

ABSTRACT
This study evaluated the potential of Ziziphus mauritiana Lam (Z. mauritiana Lam) ethanol extract improves thermal
stability and strength to prevent the growth and biofilm formation of C. albicans.The assessment of heat release
(thermal equilibrium) of acrylic resin using Differential scanning calorimetry, the strength of acrylic resin with the
universal testing machine, growth assessment of C. albicans by spectrophotometry, and biofilm assay with crystal
violet and visual images used to a microscope. Z. mauritiana Lam 12.5% provides better thermal stability with a
cooling peak enthalpy of -223.27 cal/g. In addition, concentrations of 6.25% and 3.125% at 48 hours had a better
ability to increase the strength of acrylic resin (p>0.05). Z. mauritiana Lam at all concentrations and incubation of 24
and 48 hours were similar in decreasing the growth of C. albicans (0.06-0.08 or equivalent to <300 CFU/mL).
Concentrations of 3.125% and 6.25% had a better ability to inhibit the biofilm formation of C. albicans on the acrylic
resin surface. There was no significant difference between the inhibition of growth and biofilm formation on acrylic
resin based on incubation time and concentration (p>0.05). The Z. mauritiana Lam increased heat release (thermal
stability) in acrylic resin, which caused an increase in transverse strength. At the same time, it reduces the growth and biofilm formation of C. albicans on the acrylic resin surface.

Keywords: Biofilm, Candida albicans, Growth, Denture stomatitis, Ziziphus mauritiana Lam