Effectiveness of Aspergillus sp. extract in denture adhesive on surface roughness of acrylic resin on Candida albicans biofilm formation

ABSTRACT
The denture adhesive increases retention on the denture base and affects oral microorganisms. Adding antifungals to
denture adhesives can inhibit the Candida albicans biofilms formation and prevent denture stomatitis. The combination
of denture adhesives and herbal medicines is an alternative to antifungals, which have few side effects because it is a
plant. Moreover, one of them is the endophytic Aspergillus sp. extract containing chemical compounds that can inhibit
the Candida albicans biofilms formation. This study aims to analyze the effectiveness of the endophytic Aspergillus sp.
extract in denture adhesive materials for Candida albicans biofilm formation on acrylic resin surfaces. The research
method is to extract the Aspergillus sp. extract antibiofilm test. Denture adhesive formulation was adjusted to the
standard, and added Aspergillus sp. with concentrations of 3.125%, 6.25%, 12%, and 25%. The research sample
used hot polymerized acrylic resin. The control group used X denture adhesive and added nystatin, each group
suspended by Candida albicans for 24, 48, and 72 hours. Examination of biofilm formation activity on the surface of
acrylic resin used SEM. The analysis used Two Way Anova. Aspergillus sp. extract in denture adhesive effectively
prevents Candida albicans biofilm formation within 24 hour incubation time. In conclusion, extract of the endophytic
Aspergillus sp. in denture adhesive can inhibit the formation of Candida albicans biofilm on the surface roughness of acrylic resin.

Keywords: acrylic resins; Aspergillus sp.; biofilms; Candida albicans; denture adhesives