KARAKTERISASI FTIR DAN UJI TOKSISITAS HIDROKSIAPATIT NANOKRISTALIN CORBICULA MOLTKIANA TERHADAP SEL HUMAN DERMAL FIBROBLAST ADULT

Introduction: One method for reconstructing bones is through bone grafting. Hydroxyapatite (HA) is often used as a bone graft because it has biocompatibility, osteoconductive, bioactive, non-toxic, and non-immunogenic properties. HA can be obtained from natural materials such as shells, limestone, eggshells, and others. The nanocrystalline hydroxyapatite (nHA) used in this research is from the Corbicula moltkiana shell. Aim: to know; a) FTIR characterization of nHA Corbicula moltkiana; b) the level of toxicity of Corbicula moltkiana nHA on human dermal fibroblast adult cells, and c) the differences in HDFA cell viability between concentration groups of nHA. Methods: in vitro laboratory experiment with post-test only control group design. To find out the characterization, use the FTIR characterization test. Toxicity test using the MTT method. The research samples were Corbicula moltkiana nHA powder and HDFA cells. The samples were divided into four groups: the control cell group and three groups of nHA Corbicula moltkiana with concentrations of 25 μg/ml, 50 μg/ml, and 100 μg/ml. Result: a) FTIR shows that Corbicula moltkiana nHA has all typical absorption characteristics of HA, b) Corbicula moltkiana nHA was not toxic, cell viability at concentrations of 25 μg/ml, 50 μg/ml, and 100 μg/ml against HDFA cells was 99.64%, 96.51%, and 83.93%, respectively, c)There were significant differences in each concentration group. Conclusion: Corbicula moltkiana nHA was not toxic to HDFA cells therefore this material has the potential to be used as a bone graft