In Vivo Histopathological Wound Healing in Mice(Mus Musculus) of Suruhan Extract (Peperomia Pellucida L. Kunth)

A wound is a condition where the continuity of tissue is broken, which disrupts the function and anatomical structure
of the body from the outermost surface to the deepest layer. When an injury occurs, the body will carry out a healing
process consisting of four stages: haemostasis, inflammation, proliferation, and maturation. The wound healing process can be accelerated, one of which is using a messenger plant (Peperomia pellucida) which has a
pharmacological function. The purpose of the study was to prove the difference between the administration of extracts
and 10% povidone-iodine on the histopathological score of wound healing in mice (Mus musculus) 3,5,7 and 14 days.
The type of research was true experimental with a post-test control group design. The sampling technique used is
simple random sampling. The research sample was 32 mice (Mus musculus) male sex, healthy, 2-3 months old with a
bodyweight of 20-40 g, divided into two groups, namely the treatment group and the control group where each group
consisted of 4 subgroups. The treatment group was the group that was given 10% messenger extract topically once a
day for 14 days. The control group was the group that was given 10% povidone-iodine once a day for 14 days. Skin
tissue was taken and prepared with hematoxylin-eosin staining for histopathological examination, including
epithelialization, granulation, inflammatory cells, and angiogenesis. The results of the study were analyzed by using the Mann-Whitney test. The results of the Mann-Whitney test showed p values on day 3 (p=0,200), day 5 (p=0,057),
day 7 (p=0,029) and day 14 (p=0,343). In general, there is no significant difference in histopathological scores
between the control group and the treatment group.
Keywords: Wound Healing, Wound, Histopathology Scoring, Suruhan (Peperomia Pellucida)