Molecular identification of lactic acid bacteria an approach to sustainable food security

Dessy Abdullah, Abdullah D and Sandeep Poddar, Poddar S and Ramesh Prasath Rai, Prasath Rai R and Endang Purwati, Purwati E and Nadia Purnama Dewi, Purnama Dewi N and Yudha Endra Pratama, Endra Pratama Y (2021) Molecular identification of lactic acid bacteria an approach to sustainable food security. Journal of Public Health Research, 15 (2). ISSN 2279-9036

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Abstract

Background: Dadiah is a traditional dish from West Sumatra
made from buffalo milk, which is fermented in bamboo tubesand left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB), which hasthepotential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban,Kab.Fifty Cities, West Sumatra.
Design and Methods: A survey method was used in this
research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16SrRNA gene.
Results: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zonearea results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zoneareaof23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running
PCR base length 1419bp.
Conclusions: Phylogenetic analysis shows that Dadiah of
Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Amd Regina Andina
Date Deposited: 21 Nov 2022 03:18
Last Modified: 21 Nov 2022 03:18
URI: http://repository.unbrah.ac.id/id/eprint/140

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