Sequencing Analysis of Lactic Acid Bacteria as Probiotics from Ettawah Goat's Milk as Antimicrobial

The utilization of fermented milk as
probiotics to address nutritional concerns in Indonesia has
been limited. Multiple studies have demonstrated that
probiotics can mitigate dysbiosis by altering the gut
microbiota composition. One reason is milk utilization
from domesticated animals, attributed to the nutritional
composition and presence of lactic acid bacteria (LAB) as
probiotics in goat milk. This work aimed to evaluate the
molecular potential of probiotic isolates derived from goat
milk utilizing the 16S rRNA gene and their antimicrobial
activities. This study employed the sequencing analysis of
potential probiotic isolates from goat milk using the 16S
rRNA gene. The analysis focused on examining the
arrangement of lactic acid bacteria DNA in terms of acid-
base length and evaluating their antimicrobial activity
against Escherichia coli, Salmonella sp., Klebsiella sp., as
well as their antifungal activity against Candida albicans.
The research findings were obtained by analyzing the
amplification of DNA sequences from probiotic isolates
found in goat milk, namely in a 1391 bp region.
Furthermore, the antibacterial activity was assessed by
measuring the diameter of the clear Zone formed on the test
bacteria E. coli (7.10 mm), Salmonella sp. (6.10 mm),
Klebsiella sp. (9.13 mm), and the fungus Candida albicans (15.19 mm). This study proves that LAB isolates derived
from goat milk possess probiotic properties. This is
supported by their amplification of the 16S rRNA gene,
sequencing analysis showing similarity to
Lactiplantibacillus plantarum, and their ability to inhibit
the growth of pathogenic bacteria and exhibit antifungal
activity